求英文文献（微生物类、全文）

最新问答

• 雨神的女儿

  scopus和web of science上都有全文可以下的.

  浏览 243赞 90时间 2022-10-25
• 轻清净静的美好

  只有摘要，没有全文。
  Formation of diacetyl by cell-free extracts of Leuconostoc lactis
  
  This article is not included in your organization's subscription. However, you may be able to access this article under your organization's agreement with Elsevier.
  
  Kieran N. Jordana, , , Margie O'donoghueb, Seamus Condonb and timothy M. Cogana
  
  a Teagasc, National Dairy Products Research Centre, Moorepark, Fermoy, Co. Cork, Ireland
  
  b Department of Microbiology, University College Cork, Cork, Ireland
  
  Received 28 May 1996; revised 19 July 1996; accepted 14 August 1996. ; Available online 22 March 1999.
  
  Abstract
  Diacetyl formation was linear with time and with protein concentration when a cell-free extract of Leuconostoc lactis NCW1 was added to a buffer system containing pyruvate, thiamine pyrophosphate and MgS4 (final concentrations 60 mM, 0.11 mM and 0.22 mM, respectively). No diacetyl was detected in the absence of pyruvate or cell-free extract and no increase in diacetyl formation was detected on the addition of acetyl-CoA. When 2-acetolactate (1.6 mM) was the substrate, autodecarboxylation to diacetyl and acetoin occurred under aerobic and anaerobic conditions. When cell-free extract was added, decarboxylation of 2-acetolactate to acetoin and diacetyl increased 4–6-fold, under aerobic and anaerobic conditions. When the cell-free extract was boiled, diacetyl formation from 2-acetolactate was reduced to the level of autodecarboxylation. The results suggest that diacetyl is formed enzymatically in the presence and absence of oxygen, as well as spontaneously, from 2-acetolactate.

  浏览 232赞 107时间 2022-05-27